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wet-transfer electroblotting apparatus  (Bio-Rad)


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    Structured Review

    Bio-Rad wet-transfer electroblotting apparatus
    Wet Transfer Electroblotting Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wet-transfer+electroblotting+apparatus/trans+blot+sd+semi+dry+transfer+cell/pmc03711763-62-44-47
    Average 90 stars, based on 1 article reviews
    wet-transfer electroblotting apparatus - by Bioz Stars, 2026-10
    90/100 stars

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    Article Title: Peptide Inhibitors Disrupt the Serotonin 5-HT 2C Receptor Interaction with Phosphatase and Tensin Homolog to Allosterically Modulate Cellular Signaling and Behavior
    Article Snippet: Equal amounts of protein were reduced with Laemmli’s sample buffer, heated for 20 min at 70°C, then separated by SDS-PAGE using 4–12% Bis-Tris gels (Invitrogen) for 2–3 h at 110 V. After gel electrophoresis, proteins were transferred to a PVDF membrane (Bio-Rad) via a wet-transfer electroblotting apparatus (Bio-Rad) overnight at 60 V ( Anastasio et al., 2010 ).

    Article Title: Serotonin (5-HT) 2C Receptor (5-HT 2C R) Protein Expression is Enriched in Synaptosomal and Postsynaptic Compartments of Rat Cortex
    Article Snippet: Following gel electrophoresis, proteins were transferred to a PVDF membrane (BioRad, Hercules, CA) via a wet-transfer electroblotting apparatus (BioRad) for 3 hr at 100V.

    Nucleic Acid Electrophoresis:

    Article Title: Peptide Inhibitors Disrupt the Serotonin 5-HT 2C Receptor Interaction with Phosphatase and Tensin Homolog to Allosterically Modulate Cellular Signaling and Behavior
    Article Snippet: Equal amounts of protein were reduced with Laemmli’s sample buffer, heated for 20 min at 70°C, then separated by SDS-PAGE using 4–12% Bis-Tris gels (Invitrogen) for 2–3 h at 110 V. After gel electrophoresis, proteins were transferred to a PVDF membrane (Bio-Rad) via a wet-transfer electroblotting apparatus (Bio-Rad) overnight at 60 V ( Anastasio et al., 2010 ).

    Article Title: Serotonin (5-HT) 2C Receptor (5-HT 2C R) Protein Expression is Enriched in Synaptosomal and Postsynaptic Compartments of Rat Cortex
    Article Snippet: Following gel electrophoresis, proteins were transferred to a PVDF membrane (BioRad, Hercules, CA) via a wet-transfer electroblotting apparatus (BioRad) for 3 hr at 100V.



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